Biological and Chemical Detection of Trichothecene Mycotoxins of Fusarium Species
نویسندگان
چکیده
منابع مشابه
Trichothecene mycotoxins from Fusarium culmorum cultures.
Chemical analysis of the culture filtrates of Fusarium culmorum CMI 14764 has demonstrated the presence of seven trichothecene mycotoxins. Major metabolites are 3-acetyldeoxynivalenol and 7 alpha, 8 alpha-dihydroxycalonectrin, with 3,15-diacetyldeoxynivalenol, deoxynivalenol, calonectrin, isotrichodermin and 12,13-epoxytrichothec-9-ene (EPT) as minor products. The occurrence of the rarely encou...
متن کاملBiological modification of trichothecene mycotoxins: acetylation and deacetylation of deoxynivalenols by Fusarium spp.
Attempts were made to elucidate the acetyl transformation of novel trichothecene mycotoxins, 3a,7a,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one (deoxynivalenol) and its derivatives, by trichothecene-producing strains of Fusarium nivale, F. roseum, and F. solani. In the peptone-supplemented Czapek-Dox medium, F. roseum converted 3a-acetoxy-7a,15-dihydroxy-12,13-epoxytrichothec-9-en-8-one (3-ac...
متن کاملDetection of airborne trichothecene-producing Fusarium species in chicken houses.
One hundred and forty three airborne Fusarium isolates in chicken houses belonging to seven Fusarium species were analyzed by PCR with Tri5 gene as a specific marker of mycotoxin product . The result of Tri5 gene sequence analysis indicates that the PCR amplification products were 89%-96% identical to the previously reported Tri5 genes, which were all amplified from four F. poae isolates. T-2 t...
متن کاملBiosynthesis of trichothecene mycotoxins in Fusarium culmorum cultures.
A high yielding production of the trichothecene mycotoxin 3-acetyldeoxynivalenol (3-AcDON) in cultures of Fusarium culmorum is described. By supplying [14C]acetate, 14C-labelled 3-AcDON suitable for further metabolic studies has been obtained. The pattern of labelling has been ascertained by using 13C-labelled acetate precursors, and is in line with established biosynthetic data. A second trich...
متن کاملDetection of polar and macrocyclic trichothecene mycotoxins from indoor environments.
A method is described for the qualitative and semi-quantitative simultaneous determination of both non-macrocyclic and macrocyclic trichothecene biotoxins from samples derived from indoor environments. The method includes extraction, sample pre-treatment and reversed-phase HPLC separation followed by tandem mass spectrometric identification and quantification using electrospray ionization on a ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1973
ISSN: 0003-6919
DOI: 10.1128/aem.25.4.699-704.1973